Affinity Purification

1. CNBr-activated Sepharose (APBiotech; see Note 5).

2. Sintered glass funnels.

3. Chromatography columns (APBiotech C series).

4. Peristaltic pump.

5. Concentrated HCl.

10. TBS containing 0.1% sodium azide (Sodium azide is toxic—consult MSDS data sheet).

11. TBS containing 0.1% NP40 (detergent).

12. 250 mM ethylene diamine tetraacetic acid, pH 8.0.

13. Saturated ammonium sulfate solution.

14. NP40 (detergent) stock solution: 10% in water.

17. Saline: 0.9% NaCl in distilled water.

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