1. Sepharose 4B, cyanogen bromide-activated-Sepharose, or protein A, G, or L Sepharose.
4. Cyanogen bromide (Caution: cyanogen bromide is extremely toxic, and advice should be sought on local practices regarding its safe use. The minimum amount necessary should be purchased).
6. 0.05 M Sodium phosphate, 0.1 M NaCl, pH 7.4 (see Note 5).
8. Protein A, protein G, or protein L.
9. 1 M Ethanolamine or 0.5 M glycine.
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