Phage Display Vectors

A large number of different phage display vectors have been constructed. With pretending to be complete, Table 1 lists a selection of phage display vectors. Some of them have not been used for the construction of a library up to now but have been included because they offer possible alternatives. For example, one of the systems allows the success of antibody gene cloning to be monitored by the expression of green fluorescent protein 52 . A variety of different promoters have been used for the...

The MiniPERM Bioreactor

Miniperm Bioreactor

The MiniPERM bioreactor is our method of choice for the high-density culture of hybridoma cells Fig. 1 . The modular system, consisting of a 40-mL disposable culture chamber and a 550-mL reusable nutrient module separated by a dialysis membrane 12.5 kDa molecular weight cutoff , allows for the production of a low-volume, high-density cell population with a correspondingly high antibody yield. Fig. 2. Schematic view of the MiniPERM production module. Reproduced with permission from Joachim L...