Providencia Stuartii Characteristics Alpha Hemolysis

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Staphylococcus Aureus Gram Stain

Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium (left) and from the sputum of a patient with staphylococcal pneumonia (right). The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils.

Gram Stain Sputum Gpc Chain

Plate 2 Streptococcus pyogenes in Gram-stained smears. From a culture plate (left), the gram-positive organisms appear singly, in chains, and in clumps. In broth culture media (center), the characteristic long chains are seen. In a smear from an abscess (right), the organisms are primarily gram-positive cocci in long chains.

Direct Microscopic Smear Listeria

Plate 3 Streptococcus pneumoniae in Gram-stained smears. The organisms from a colony growing on agar medium (left) are gram positive and lancet shaped and appear in pairs and short chains. In a Gram stain of cerebrospinal fluid from a patient with pneumococcal meningitis (right), the organisms are mostly diplococci. The capsule (arrow) can be seen around some bacteria, outlined by the pink proteinaceous material of the fluid.

Plate 4 Neisseria gonorrhoeae in a Gram-stained smear from a male urethral exudate appear as gramnegative, bean-shaped diplococci.

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Klebsiella Pneumoniae Tsi

Plate 5 Gram-negative bacilli (Klebsiella pneumoniae) in a Gram-stained smear from an agar colony (left) and a patient's blood culture (right). In the blood specimen, the organisms are pleomorphic, varying in length from coccobacillary to filamentous.

Providencia AlcalifaciensEndospore Staining Stuartii

Plate 8 Bacillus spp. are gram-positive bacilli with endospores. Endospores appear as clear areas within the vegetative bacterial cell (arrows).

Plate 6 Curved, spiral, gram-negative bacilli (Campylobacter jejuni) in a Gram stain from culture. Some bacteria line up to form spirilla like chains. Courtesy Dr. E. J. Bottone

Plate 7 Spirochetes ('Treponema pallidum) appear black (arrows) in a skin preparation stained with a silver stain. Courtesy Dr. E. J. Bottone

Plate 9 Mycobacterium tuberculosis in an acid-fast stain of sputum (left). The acid-fast bacilli appear as red, beaded rods against a blue background (x 1,000). Strains of M. tuberculosis often form ropy "cords" (center), which is considered an indication of virulence. When stained with a fluorescent dye, acid-fast bacilli fluoresce brightly against a dark background (right, x400).

Plate 10 The quellung reaction. The halo around the cells is the pneumococcal capsule, which appears to swell when the cells are treated with pneumococcal antiserum. Courtesy Dr. E. J. Bottone

Providencia Stuartii Hemolysis

Plate 11 Streptococcus pyogenes growing on a blood agar plate. The clear beta-hemolytic areas surrounding the punctate colonies are caused by a streptolysin enzyme.

Punctate Colonies

Plate 12 The large capsule of Klebsiella pneumoniae gives a mucoid appearance to colonies growing on agar plates. Courtesy

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Providencia StuartiiNeisseria Gonorrhoeae Chocolate Agar

Plate 13 Neisseria gonorrhoeae colonies on chocolate agar. The oxidase-positive organisms become deep purple when a drop of oxidase reagent is added to an area of the plate (right).

Plate 14 A disk diffusion antimicrobial susceptibility test. If the clear zones of growth inhibition around disks are of a certain diameter, the organism is susceptible to the antimicrobial agent in the disk.

Green Fluorescent Pigment Pseudomonas

Plate 15 A microdilution susceptibility test of Pseudomonas aeruginosa. The green color signifies growth of the organism with production of its soluble green pigment. Growth occurs in the wells containing concentrations of antimicrobial agents to which the organism is resistant.

Plate 16 A MacConkey agar plate with Escherichia coli (pink, lactose-fermenting colonies) growing on the left-hand side and a Salmonella sp. (colorless, lactose nonfermenting colonies) on the right. Courtesy Dr. E. J. Bottone.

Plates With Providencia StuartiiH2o2 Tsi Salmonella

Plate 17 Escherichia coli (left) and Salmonella sp. (right) on a Hektoen enteric agar plate. The lactose-fermenting E. coli colonies appear yellow, whereas the Salmonella colonies appear black because of hydrogen sulfide production. Compare with reactions on colorplate 16 and note how selective and differential media display different organism characteristics.

Plate 18 When Durham tubes are placed inside broth tubes, gas produced by fermentation of carbohydrates in the medium can be visualized as a bubble in the inner tube (left). The organism on the right does not produce gas when fermenting carbohydrates.

Plate 19 Kligler iron agar slants test for fermentation of glucose and lactose and the production of gas and hydrogen sulfide. The organism on the left ferments both glucose and lactose with gas production (bubbles in medium). The organism in the middle ferments glucose (yellow butt) but not lactose (pink slant). The organism on the right ferments glucose (with gas production) but not lactose, and blackens the agar as a result of hydrogen sulfide production. Reactions are similar on TSI slants.

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Tsi Slant ColiProvidencia Stuartii Slant

Plate 20 Urease test. The organism on the right produces the enzyme urease, which imparts the bright pink alkaline reaction to the urea agar slant.

Courtesy Dr. E. J. Bottone.

Plate 21 DNase test. When a plate containing DNA is flooded with toluidine blue, the colony of deoxyribonuclease-producing organisms (top) and the surrounding area of hydrolyzed DNA become pink.

Legionella Blue Latex Results

Plate 23 Fluorescent antibody preparation of Legionella pneumophila viewed microscopically with an ultraviolet light source. After the patient specimen is treated with an antibody conjugated with a fluorescent dye, the brightly fluorescing bacilli are easily visible against the dark background.

Plate 24 Latex agglutination reaction. Antibody-coated latex particles have been mixed with the positive (well 1) and negative (well 2) controls and the organism isolated from the patient (well 3). The dark blue rims of the positive control and patient mixtures represent the positive reaction of agglutinated latex particles. Well 6 is an additional control.

Phenylalanine Deaminase Test

Plate 22 Phenylalanine deaminase (PDase) test. The PDase-producing Providencia stuartii (left) hydrolyzes phenylalanine in the culture medium. After ferric chloride is added to the slant, the green positive reaction appears. In the middle is the PDase-negative Escherichia coli; the tube on the right is uninoculated.

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Slant Test Tube Staph Saprophyticus

Plate 26 Coagulase test. The tube of plasma on the right was inoculated with Staphylococcus aureus. A solid clot has formed in this tube in comparison to the still liquid plasma in the uninoculated tube on the left. Courtesy Dr. E. J. Bottone.

Plate 25 A direct enzyme immunoassay for Clostridium difficile toxin. The wells have been coated with antibody against the toxin and suspensions of patient fecal specimens added. The first well in row A and the second wells in rows G and H are strongly positive, whereas the second well in row E shows a weakly positive reaction. In the third column, positive (row A) and negative (row B) controls are shown. Refer to figure 19.3 for details of the test.

Plate 27 Novobiocin disk test for differentiating two coagulase-negative species of staphylococci: Staphylococcus saprophyticus (left) and Staphylococcus epidermidis (right). The zone of inhibition around S. saprophyticus is less than 16 mm, which identifies this species by its resistance to the antibiotic.

Saprophyticus Morphology

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Plate 28 Subsurface colonies of alpha- (left), beta- (center), and nonhemolytic (right) streptococci. Note many intact red cells and a greenish color around the alpha-hemolytic colonies. Hemolysins produced by beta-hemolytic colonies have completely destroyed surrounding red cells. Nonhemolytic organisms produce no change in the red cells.

Providencia Spp ColoniesProvidencia Stuartii

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Responses

  • ZEWDI YOHANNES
    Does pseudomonas aeruginosa produce urease?
    8 years ago
  • karita
    What does bacteria like salmonella and e. coli look like on TSI slants?
    8 years ago
  • Tiziana Lettiere
    Is providencia stuartii positive for phenylalanine deaminase?
    8 years ago
  • Debbie
    What does a capsule stain of klebsiella pneumoniae look like?
    8 years ago
  • BRUNO
    Is providencia stuartii acid fast?
    8 years ago
  • robert
    What does providencia species look like on macconkey?
    8 years ago
  • paula
    Is e coli and providencia stuartii phenylalanine deaminase positive?
    8 years ago
  • stella
    Is Providiencia stuartii lactose positive?
    8 years ago
  • Jodi Granderson
    What is the gram reaction and morphology of providencia stuartii?
    7 years ago
  • belladonna
    How toe grow neisseria gonorrohea in lab?
    7 years ago
  • Tyko
    Can providencia rettgeri be beta hemolytic?
    7 years ago
  • Muhammed
    What are the colony morphology of providencia stuartii?
    7 years ago
  • pervinca
    Is providencia stuartii a capsule?
    7 years ago
  • SIMRET
    Does providencia stuartii test negative for hydrogen sulfide test?
    7 years ago
  • Elanor Rumble
    What does providencia stuartii look like on a streak plate?
    7 years ago
  • richard
    Is proventeia stuartii lactose negative on macconkey agar?
    7 years ago
  • SEBHAT EYOB
    Which plate does klebsiella?
    7 years ago
  • wolfgang
    Is providencia rettgeri cocci or bacillus?
    6 years ago
  • oberto
    Is a culture of 50000 alot for providencia stuartii?
    6 years ago
  • ermenegilda
    What is providencia stuartii an acid fast stain?
    6 years ago
  • Lucius
    Does providencia stuartii grow on HE media?
    6 years ago
  • maya
    Does providencia produce hemolysis on blood agar?
    5 years ago
  • yvonne
    Is providencia stuartii Phenylalanine Deaminase positive or negative?
    5 years ago
  • loredana
    Is providencia stuartii negative for H2S production?
    4 years ago
  • Adalberto
    What is colony morphology and growth characteristics of Providencia stuartii in broth?
    4 years ago
  • robert gonsalves
    Does providencia look black on hektoen agar?
    4 years ago
  • james
    Does providence stuartii pigmnet test?
    4 years ago
  • Adelard
    Is salmonella sp an acidfast stain?
    4 years ago
  • Jaiden
    Does providencia stuartii grow on emb agar?
    3 years ago
  • mario
    Is providencia species a beta haemolytic?
    3 years ago
  • Susie
    Does bacteria providencia stuartii ferment mannitol?
    3 years ago
  • henriikka
    Does p stuartii ferment glucose?
    3 years ago
  • monika
    Does p. staurtii lactose ferment?
    3 years ago
  • demsas
    Does providencia have capsules?
    2 years ago
  • donnie
    Is providencia stuartii acid fast positive?
    2 years ago
  • bilbo
    Does providencia stuartii produce phenylalanine deaminase?
    1 year ago
  • pietro
    Does p stuartii produce gas or acid in fermentation?
    12 months ago
  • nazzareno
    Is Providencia alpha hemolytic?
    12 months ago
  • tiia hurme
    Does providencia stuartii smell?
    11 months ago
  • Diamanda
    Does treponema ferment lactose?
    10 months ago
  • kaarina
    Does providencia stuartii swarm on blood agar?
    9 months ago
  • tanja
    Can providencia be a lactose fermenter?
    8 months ago
  • IBRAHIM
    What does p. stuartii look like when you do a triple sugar iron test?
    6 months ago
  • ralf
    Does providencia stuartii ferment lactose?
    5 months ago
  • frodo
    What hemolysis does P. ALCALIFACIENS do in sba?
    4 months ago

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