Workbook in Microbiology Laboratory Techniques 7e

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Table 31.2 Some Important Pathogenic Fungi

Organisms

Morphological Features

Yeasts or yeastlike

Cryptococcus neoformans

Yeasty soft colonies

Encapsulated budding cells

Candida albicans

Budding cells, pseudomycelium, and

chlamydospores

Systemic fungi

Histoplasma capsulatum

In tissues, intracellular and yeastlike

In culture at 37°C, a yeast

In culture at room temperature, a mold with

characteristic macroconidia (spores)

Coccidioides immitis

In tissues, produces spherules filled with

endospores

In culture, a cottony mold with fragmenting

mycelium

Blastomyces dermatitidis

In tissues, a large thick-walled budding yeast

In culture at 37°C, a yeast

In culture at room temperature, a mold

Paracoccidioides brasiliensis

In tissues, a large yeast showing multiple budding

In culture at 37°C, a multiple budding yeast

In culture at room temperature, a mold

Subcutaneous fungi

Sporothrix schenckii

In tissues, a small gram-positive, spindle-shaped

yeast

In culture at 37°C, a yeast

In culture at room temperature, a mold with

characteristic spores

Cladosporium

In tissues, dark, thick-walled septate bodies

Fonsecaea >

In culture, darkly pigmented molds

Phialophora

Madurella

Tissue and culture forms vary with causative fungus

Pseudallescheria >

Curvularia and others J

Superficial fungi

Microsporum species

These fungi grow in cultures incubated at room

Trichophyton species

temperatures as molds, distinguished by the

Epidermophyton floccosum

morphology of their reproductive spores

Pneumonia, meningitis, other tissue infections Skin and mucosal infections, sometimes systemic

Histoplasmosis is primarily a disease of the lungs; may progress through the mononuclear phagocyte system to other organs

Coccidioidomycosis is usually a respiratory disease; may become disseminated and progressive

Sporotrichosis is a local infection of injured subcutaneous tissues and regional lymph nodes

Ringworm of the scalp, body, feet, or nails

Diseases

Pneumonia, meningitis, other tissue infections Skin and mucosal infections, sometimes systemic

Histoplasmosis is primarily a disease of the lungs; may progress through the mononuclear phagocyte system to other organs

Coccidioidomycosis is usually a respiratory disease; may become disseminated and progressive

North American blastomycosis is an infection that may involve lungs, skin, or bones

Paracoccidioidomycosis (South American blastomycosis) is a pulmonary disease that may become disseminated to mucocutaneous membranes, lymph nodes, or skin

Sporotrichosis is a local infection of injured subcutaneous tissues and regional lymph nodes

Chromoblastomycosis is an infection of skin and lymphatics of the extremities caused by any one of several species

Mycetoma (maduromycosis, madura foot) is an infection of subcutaneous tissues, usually of the foot, caused by any one of several species

Ringworm of the scalp, body, feet, or nails

5. Make a drawing of the yeast cells that you see with and without germ tubes.

6. Make a Gram stain of the Candida culture and draw your observations.

7. Prepare a transparent tape preparation of the Aspergillus, Rhhizopus, and Penicillium growth as follows (see figure 31.1): Place a drop of lactophenol cotton blue on a clean microscope slide. Carefully uncover a plate culture of one of the molds. Cut a piece of transparent tape slightly longer than the length of the microscope slide (about 4 inches). Hold the tape in a U shape with the sticky side down and gently touch the surface of a mold colony. Some of the colony growth will adhere to the tape. Place the tape with the sticky side down across the microscope slide so that the colony growth is in contact with

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Cure Your Yeast Infection For Good

Cure Your Yeast Infection For Good

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