pass through bacterial filters, they do not stain with ordinary dyes, and they are resistant to antimicrobial agents (such as penicillin) that act by interfering with cell wall synthesis.
These organisms can be cultivated on enriched culture media, but on agar media their colonies can be clearly seen only with magnifying lenses. They do not heap on the surface, but extend into and through the agar from the point of inoculation.
Specimens for laboratory diagnosis include sputum, urethral or cervical discharge, synovial fluid, or any material from the site of suspected infection. Cultures require 3 to 10 days of incubation at 35°C. Serological methods are also available for detecting mycoplasmal antibodies in the patient's serum.
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