Workbook in Microbiology 7e

4. Why is culture the standard method for diagnosing gonorrhea in possible medical-legal cases?

5. How are pathogenic Neisseria identified?

6. Name two common causes of bacterial meningitis.

7. When spinal fluid is collected for laboratory diagnosis of meningitis, how should it be transported?

8. Where are Neisseria found as normal flora? Treponema?

9. Name the etiologic agents of syphilis, leptospirosis, and Lyme disease.

10. Can T. pallidum be demonstrated by Gram stain? If not, what technique would you use to view this organism microscopically?

11. What is the importance of the laboratory diagnosis of syphilis and gonorrhea?

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Workbook in Microbiology, 7/e

12. Complete the following table.



Gram-Stain Reaction

Microscopic Morphology

Laboratory Specimens

Diagnosis Method

N. gonorrhoeae

N. meningitidis

T. pallidum

B. burgdorferi

L. interrogans

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Workbook in Microbiology, Laboratory Techniques 7/e


Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients' Antibodies

In Sections VIII through X, we have studied microbiological techniques for isolating and identifying aerobic or facultatively anaerobic bacteria. In this section we shall see how anaerobic bacteria are cultivated. The general techniques for identifying mycobacteria and microbial pathogens of other types (fungi, viruses, animal parasites) are also described in these exercises. In some infections, evidence of the causative microorganism is obtained by examining the patient's serum for specific antibodies against the suspected pathogen. This is usually done when the microorganisms are difficult to cultivate by routine methods or sufficient time has elapsed such that the organism is no longer recoverable in culture from patient specimens.

You should note that these techniques are quite varied. It is therefore important to remember that when specimens are ordered for laboratory diagnosis of mi-crobial disease, the suspected clinical diagnosis should be stated on the request slip or in the computer so that appropriate laboratory procedures can be instituted promptly.

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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