Record and diagram your observations.
Describe the arrangement of flagella you observed:
1. Why must special stains be used to visualize bacterial capsules, flagella, and endospores?
2. Why is it important to know whether or not bacterial cells possess capsules, flagella, or endospores?
3. What do endospore stains have in common with the Ziehl-Neelsen acid-fast stain?
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4. Is bacterial sporulation a reproductive process? Explain.
5. Why is it important to determine the location of the endospore within the bacterial cell?
6. Can you relate endospore staining to endospore survival in hospital or other environments?
7. What is a negative stain?
8. Describe a flagella stain and explain the principle of its action.
9. Compare the usefulness of a flagella stain with that of the hanging-drop or wet-mount preparation.
10. What is the quellung reaction? How might it be used to rapidly identify certain bacteria directly in clinical specimens?
11. Of what value is a capsule to a microorganism?
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I. Basic Techniques of Microbiology
Cultivation of Microorganisms
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