Image Of Positive Catalase Test

1. Divide a clean glass slide into two sections with your marking pen or pencil.

2. With a sterilized and cooled inoculating loop, pick up a small amount of the Staphylococcus culture from the nutrient agar slant. Smear the culture directly onto the left-hand side of the slide. The smear should be about the size of a pea.

3. Sterilize the loop again and smear a small amount of the Enterococcus culture on the right-hand side of the slide.

4. With the capillary pipette, place one drop of hydrogen peroxide over each smear. Be careful not to run the drops together. Observe the fluid over the smears for the appearance of gas bubbles (see fig. 18.1). Record the results in the chart. Discard the slide in a jar of disinfectant.

Figure 18.1 Slide catalase test. Staphylococcus epidermidis on the left produces a strong positive catalase reaction.

Enterococcus faecalis on the right (cloudy area in drop of hydrogen peroxide) is negative in the catalase test.

Figure 18.1 Slide catalase test. Staphylococcus epidermidis on the left produces a strong positive catalase reaction.

Enterococcus faecalis on the right (cloudy area in drop of hydrogen peroxide) is negative in the catalase test.

Enterococcus Catalase Test

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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