1. Bring a fresh sample of your feces to the laboratory session. Collect it in a clean container fitted with a tight lid (a screw-cap jar; waxed, cardboard cup; or plastic vessel).
2. Using a swab, take up about 1 gm of feces (a piece the size of a large pea) and emulsify this in the tube of saline.
3. Inoculate the fecal suspension, with the swab, on a blood agar, EMB or MacConkey agar plate, and a Hektoen enteric (HE) plate. Discard the swab in disinfectant solution. Streak for isolation, using a loop.
4. Incubate the plates at 35°C for 24 hours.
Was this article helpful?