1. Place two small drops of water or saline on the surface of a clean glass slide.

2. Pick up a beta-lactamase disk with your forceps and place it in contact with one drop of fluid.

3. Repeat this procedure with a second disk, placing it on the second drop of fluid. Do not oversaturate the disks.

4. With your sterilized and cooled inoculating loop, pick up a portion of a B. subtilis colony and rub it across the surface of the first disk.

5. Rub a portion of a S. aureus colony across the surface of the second disk.

6. Observe the areas on the beta-lactamase disks where the organisms were inoculated for up to 30 minutes. A positive result is usually seen within 3 to 4 minutes.

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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