1. Place a swab in a nutrient broth to moisten it. As you withdraw the swab, press it against the inner wall of the tube to drain off excess fluid.
2. Take a culture of the floor with this swab by rubbing and rotating it over an area approximately 10 cm square.
4. Moisten another swab in broth and take a culture of the sink faucet in the area around the aerator or strainer. Inoculate and streak another agar plate as in step 3.
5. Take a fresh agar plate and touch separate areas of the agar surface with each fingertip of your right hand.
6. Take an agar plate into the lavatory. Place it on a shelf or the basin, remove the top, and leave the agar exposed for 30 minutes. Close, invert, and incubate the plate at 35°C.
7. Look around the laboratory for any area where dust has accumulated (window ledges, open shelves, hard-to-clean areas). Take a culture of dust with a moist swab, inoculate, and streak an agar plate.
8. Take a culture (with a moist swab) of a 5-cm square area on the front of your laboratory coat. Inoculate and streak a plate.
9. Run a moist swab through your hair. Inoculate and streak a plate.
10. Incubate all plates, inverted, at 35°C.
Morello-Mizer-Granato: I I. Basic Techniques of I 4. Cultivation of I I © The McGraw-Hill
Laboratory Manual and Microbiology Microorganisms Companies, 2003 Workbook in Microbiology, 7/e
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