Procedures

1. Place a swab in a nutrient broth to moisten it. As you withdraw the swab, press it against the inner wall of the tube to drain off excess fluid.

2. Take a culture of the floor with this swab by rubbing and rotating it over an area approximately 10 cm square.

3. Inoculate an agar plate with the swab by rotating it over a small area near one edge. Discard the swab and use your wire loop to streak out the plate in a manner to obtain isolated colonies.

4. Moisten another swab in broth and take a culture of the sink faucet in the area around the aerator or strainer. Inoculate and streak another agar plate as in step 3.

5. Take a fresh agar plate and touch separate areas of the agar surface with each fingertip of your right hand.

6. Take an agar plate into the lavatory. Place it on a shelf or the basin, remove the top, and leave the agar exposed for 30 minutes. Close, invert, and incubate the plate at 35°C.

7. Look around the laboratory for any area where dust has accumulated (window ledges, open shelves, hard-to-clean areas). Take a culture of dust with a moist swab, inoculate, and streak an agar plate.

8. Take a culture (with a moist swab) of a 5-cm square area on the front of your laboratory coat. Inoculate and streak a plate.

9. Run a moist swab through your hair. Inoculate and streak a plate.

10. Incubate all plates, inverted, at 35°C.

Morello-Mizer-Granato: I I. Basic Techniques of I 4. Cultivation of I I © The McGraw-Hill

Laboratory Manual and Microbiology Microorganisms Companies, 2003 Workbook in Microbiology, 7/e

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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