How To Prepare A Wet Mount

1. Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide (fig. 3.2, step 1).

2. Carefully place a clean cover glass (see Experiment 3.1, procedure 1) over the drop, trying to avoid bubble formation (fig. 3.2, step 2). The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing.

3. If you examine the slide immediately, you need not seal the coverslip. Otherwise, seal around the edges of the coverslip with a thin film of clear nail polish (fig. 3.2, step 3). Be certain the nail polish is completely dry before examining the slide under the microscope.

4. Examine the preparation in the same manner as in Experiment 3.1, following procedures 6 through 10. Instead of focusing on the edge of the drop, however, you may find it helpful to focus first on the left-hand edge of the coverslip.

5. Make a wet-mount preparation of the Staphylococcus culture, following the same procedures just described.

Figure 3.2 Wet-mount preparation.

Wet Mount Procedure

Morello-Mizer-Granato: I I. Basic Techniques of I 2. Microscopic I I © The McGraw-Hill

Laboratory Manual and Microbiology Morphology of Companies, 2003

Workbook in Microbiology, Microorganisms 7/e

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  • josh
    How to make a wet mount?
    8 years ago
  • neve
    How to make wet mounts?
    8 years ago
  • selamawit
    How to make a wet mount slide?
    8 years ago
  • Tony
    How to Prepare a Wet Mount:?
    8 years ago
  • Karly
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    How to make a wet mound?
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    8 years ago
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    8 years ago
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    How to make preparation of microscopik egzamination?
    7 years ago
  • cheryl
    How to seal coverslips with nail polish?
    7 years ago
  • Heather Earl
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    7 years ago
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    7 years ago
  • donato cremonesi
    How to make a microscope slide with nail polish?
    6 years ago
  • cynthia kirkland
    What is wet films in microbiology?
    3 years ago
  • yemane
    How to prepare a wet mount with urine?
    1 year ago
  • Teodros
    How to prepare a simple urine slide?
    7 months ago
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    7 months ago
  • haben
    How is a wet mount slide prepared?
    6 months ago
  • D
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    6 months ago
  • cora burrows
    How to seal coverslip with nail polish?
    5 months ago
  • patrice
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    10 days ago

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