From the Intestinal Tract

This exercise provides you with an opportunity to apply your knowledge of the Enterobacteriaceae to making a laboratory diagnosis of an intestinal infection. In Experiment 25.1 you will prepare a culture of your own feces and observe the normal intestinal flora on primary isolation plates. In Experiment 25.2 you will be given a pure culture of one of the Enterobacteriaceae as an "unknown" to be identified. Experiment 25.3 is an antimicrobial susceptibility test of your pure unknown culture. Here you should observe the differences in response of gram-negative enteric bacilli, as compared with streptococci and staphylococci studied earlier, to the most clinically useful antimicrobial agents.

EXPERIMENT 25.1 Culturing a Fecal Sample

Purpose

To study some enteric bacilli normally found in the bowel

Materials

A stool specimen

Swab

Tubed sterile saline (0.5 ml)

EMB or MacConkey agar plate

Hektoen enteric (HE) agar plate

Blood agar plate

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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