Experiment 212 The CAMP Test for Group B Streptococci

Group B streptococci can be distinguished from other beta-hemolytic streptococci by their production of a substance called the CAMP factor. This term is an acronym for the names of the investigators who first described the factor: Christie, Atkins, and Munch-Petersen. The substance is a peptide that acts together with the beta-hemolysin produced by some strains of Staphylococcus aureus, enhancing the effect of the latter on a sheep blood agar plate. This effect is sometimes referred to as synergistic hemolysis (see colorplate 30).

Purpose

To differentiate group B from group A streptococci by the effect of the group B CAMP factor and

by a serological method

Materials

Demonstration sheep blood agar plate, streaked at separate points with Staphylococcus aureus, group B

streptococci, and group A streptococci

Solution with extracted antigen of beta-hemolytic Streptococcus (prepared by instructor)

Latex test kit for serological typing

Morello-Mizer-Granato: I III. Diagnostic I 8. Microbiology of the I I © The McGraw-Hill

Laboratory Manual and Microbiology in Action Respiratory Tract Companies, 2003 Workbook in Microbiology, 7/e

Was this article helpful?

0 0
Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

Get My Free Ebook


Post a comment