Workbook in Microbiology 7e

Microbiology Mold Slides

And debris, making the fungi more prominent. Slides must be examined with reduced illumination to allow fungal structures to be seen (see colorplate 47). 2. Calcofluor white This reagent is used with most specimen types to detect the presence of fungi by fluorescence microscopy. The cell walls of the fungi bind the stain and fluoresce blue-white or apple green, depending on the filter combination used with the microscope. This stain is useful for examining skin scrapings for the presence of...

How To Prepare A Wet Mount

Wet Mount Procedure

Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide fig. 3.2, step 1 . 2. Carefully place a clean cover glass see Experiment 3.1, procedure 1 over the drop, trying to avoid bubble formation fig. 3.2, step 2 . The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing. 3. If you examine the slide immediately, you need not seal the coverslip....

Providencia Stuartii Characteristics Alpha Hemolysis

Providencia Alcalifaciens

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

Exercise 2Handling and Examining Cultures

Growth Patterns Broths

Microscopic examination of microorganisms provides important information about their morphology but does not tell us much about their biological characteristics. To obtain such information, we need to observe microorganisms in culture. If we are to cultivate them successfully in the laboratory, we must provide them with suitable nutrients, such as protein components, carbohydrates, minerals, vitamins, and moisture in the right composition. This mixture is called a culture medium plural, media ....

Laboratory Manual andMicrobiology in Action Intestinal Tract Companies 2003 Workbook in Microbiology 7e

One type of kit, the Enterotube II BD Diagnostic Systems , is a tube of 12 compartmentalized, conventional agar media that can be inoculated rapidly from a single isolated colony on an agar plate see colorplate 36 . The media provided indicate whether the organism ferments the carbohydrates glucose, lactose, adonitol, arabinose, sorbitol, and dulcitol produces H2S and or indole produces acetylmethylcarbinol deaminates phenylalanine splits urea decarboxylates lysine and or ornithine and can use...