Workbook in Microbiology 7e

Microbiology Mold Slides

And debris, making the fungi more prominent. Slides must be examined with reduced illumination to allow fungal structures to be seen (see colorplate 47). 2. Calcofluor white This reagent is used with most specimen types to detect the presence of fungi by fluorescence microscopy. The cell walls of the fungi bind the stain and fluoresce blue-white or apple green, depending on the filter combination used with the microscope. This stain is useful for examining skin scrapings for the presence of...

Candle Jar Microbiology

Microbiology Candle Jar

Record your observations of the colonial and microscopic morphology of pneumococci and other alpha-hemolytic streptococci in the table following step 3 on page 159. 2. Record results of the bile solubility test in the table. Describe here the appearance of each tube at the end of the 15-minute test. Alpha-hemolytic Streptococcus suspension with bile _ Morello-Mizer-Granato I III. Diagnostic I 8. Microbiology of the I I The McGraw-Hill Laboratory Manual and Microbiology in Action Respiratory...

Exercise 2 Neisseria and Spirochetes

Microbiology Neisseria

The sexually transmitted diseases are perhaps the most important infections acquired through the urogenital tract, from the social as well as medical points of view. Three frequent infectious diseases of this type are gonorrhea, syphilis, and chlamydial urethritis cervicitis. All three infections are caused by bacteria. Gonorrhea is caused by Neisseria gonorrhoeae syphilis by Treponema pallidum, a spirochete and chlamydial infection by Chlamydia trachomatis. Neisseria gonorrhoeae can be grown...

How To Prepare A Wet Mount

Wet Mount Procedure

Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide fig. 3.2, step 1 . 2. Carefully place a clean cover glass see Experiment 3.1, procedure 1 over the drop, trying to avoid bubble formation fig. 3.2, step 2 . The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing. 3. If you examine the slide immediately, you need not seal the coverslip....

Providencia Stuartii Characteristics Alpha Hemolysis

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

When Is It Appropriate To Ask The Laboratory To Perform An Acid-fast Stain

Prepare two fixed smears of each culture and two of the simulated sputum. In practice, the smears are fixed with methanol for one minute or are heat-fixed at 65 to 75 C to be certain any tuberculosis bacilli present are killed. To make smears of the agar slant culture, first place a drop of water on the slide, and then emulsify a small amount of the colonial growth in this drop. 2. Ring and code one slide of each pair with your marking pencil or pen, as usual. 3. The other slide of each pair...