Workbook in Microbiology 7e

Microbiology Mold Slides

And debris, making the fungi more prominent. Slides must be examined with reduced illumination to allow fungal structures to be seen (see colorplate 47). 2. Calcofluor white This reagent is used with most specimen types to detect the presence of fungi by fluorescence microscopy. The cell walls of the fungi bind the stain and fluoresce blue-white or apple green, depending on the filter combination used with the microscope. This stain is useful for examining skin scrapings for the presence of...

Exercise 2 Neisseria and Spirochetes

Microbiology Neisseria

The sexually transmitted diseases are perhaps the most important infections acquired through the urogenital tract, from the social as well as medical points of view. Three frequent infectious diseases of this type are gonorrhea, syphilis, and chlamydial urethritis cervicitis. All three infections are caused by bacteria. Gonorrhea is caused by Neisseria gonorrhoeae syphilis by Treponema pallidum, a spirochete and chlamydial infection by Chlamydia trachomatis. Neisseria gonorrhoeae can be grown...

Experiment 201 Isolation and Identification of Staphylococci

The laboratory diagnosis of staphylococcal disease is made by identifying the organism usually S. aureus in a clinical specimen representing the site of infection pus from a skin lesion, sputum when pneumonia is suspected, urine, spinal fluid, or blood . It should be remembered that either S. aureus or S. epidemidis may be harmlessly present on superficial tissues. Special care must be taken not to contaminate the specimen with normal flora, and laboratory results must be interpreted in the...

How To Prepare A Wet Mount

Wet Mount Procedure

Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide fig. 3.2, step 1 . 2. Carefully place a clean cover glass see Experiment 3.1, procedure 1 over the drop, trying to avoid bubble formation fig. 3.2, step 2 . The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing. 3. If you examine the slide immediately, you need not seal the coverslip....

Providencia Stuartii Characteristics Alpha Hemolysis

Staphylococcus Aureus Gram Stain

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

Is Bacterial Sporulation A Reproductive Process Explain

Bacterial Capsule Images

Record and diagram your observations. Describe the arrangement of flagella you observed 1. Why must special stains be used to visualize bacterial capsules, flagella, and endospores 2. Why is it important to know whether or not bacterial cells possess capsules, flagella, or endospores 3. What do endospore stains have in common with the Ziehl-Neelsen acid-fast stain Morello-Mizer-Granato I I. Basic Techniques of I 3. Differential Stains I I The McGraw-Hill Laboratory Manual and Microbiology...

Simple Stains

Procedure Microbiological Specimens

As we have seen in Exercise 3, wet mounts of bacterial cultures can be very informative, but they have limitations. Bacteria bounce about in fluid suspensions with Brownian movement or true motility, and are difficult to visualize sharply. We can see their shapes and observe their activity under a cover glass, but it is difficult to form a complete idea of their morphology. An important part of the problem is the minute size of bacteria. Because they are so small and have so little substance,...