And Bordetella

The genus Haemophilus contains a number of species of fastidious, gram-negative bacilli. Most of these are found as normal flora of the upper respiratory tract. Haemophilus species can cause infections in a variety of sites in the upper respiratory tract and elsewhere in the body. Laboratory diagnosis is made by identifying these organisms in clinical specimens appropriately representing the area of infection (throat swab, sinus drainage, sputum, conjunctival swab, spinal fluid, blood, or...

Workbook in Microbiology 7e

Exercise Fungi Yeasts and Molds Medical mycology is concerned with the study and identification of the pathogenic yeasts and molds, collectively called fungi (sing., fungus). You should be familiar with a number of important mycotic diseases. Yeasts are unicellular fungi that reproduce by budding, that is, by forming and pinching off daughter cells (see colorplate 41). Yeast cells are much larger (about five to eight times) than bacterial cells. The best-known (and most useful) species is...

Exercise 2 Neisseria and Spirochetes

Microbiology Neisseria

The sexually transmitted diseases are perhaps the most important infections acquired through the urogenital tract, from the social as well as medical points of view. Three frequent infectious diseases of this type are gonorrhea, syphilis, and chlamydial urethritis cervicitis. All three infections are caused by bacteria. Gonorrhea is caused by Neisseria gonorrhoeae syphilis by Treponema pallidum, a spirochete and chlamydial infection by Chlamydia trachomatis. Neisseria gonorrhoeae can be grown...

How To Prepare A Wet Mount

Wet Mount Procedure

Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide fig. 3.2, step 1 . 2. Carefully place a clean cover glass see Experiment 3.1, procedure 1 over the drop, trying to avoid bubble formation fig. 3.2, step 2 . The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing. 3. If you examine the slide immediately, you need not seal the coverslip....

Providencia Stuartii Characteristics Alpha Hemolysis

Klebsiella Pneumoniae Tsi

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

Is Bacterial Sporulation A Reproductive Process Explain

Bacterial Capsule Images

Record and diagram your observations. Describe the arrangement of flagella you observed 1. Why must special stains be used to visualize bacterial capsules, flagella, and endospores 2. Why is it important to know whether or not bacterial cells possess capsules, flagella, or endospores 3. What do endospore stains have in common with the Ziehl-Neelsen acid-fast stain Morello-Mizer-Granato I I. Basic Techniques of I 3. Differential Stains I I The McGraw-Hill Laboratory Manual and Microbiology...

Simple Stains

Procedure Microbiological Specimens

As we have seen in Exercise 3, wet mounts of bacterial cultures can be very informative, but they have limitations. Bacteria bounce about in fluid suspensions with Brownian movement or true motility, and are difficult to visualize sharply. We can see their shapes and observe their activity under a cover glass, but it is difficult to form a complete idea of their morphology. An important part of the problem is the minute size of bacteria. Because they are so small and have so little substance,...