Workbook in Microbiology 7e

Microbiology Mold Slides

And debris, making the fungi more prominent. Slides must be examined with reduced illumination to allow fungal structures to be seen (see colorplate 47). 2. Calcofluor white This reagent is used with most specimen types to detect the presence of fungi by fluorescence microscopy. The cell walls of the fungi bind the stain and fluoresce blue-white or apple green, depending on the filter combination used with the microscope. This stain is useful for examining skin scrapings for the presence of...

How To Prepare A Wet Mount

Wet Mount Procedure

Using a pipette bulb, aspirate a small amount of the Proteus culture with a capillary pipette and place a small drop on a clean microscope slide fig. 3.2, step 1 . 2. Carefully place a clean cover glass see Experiment 3.1, procedure 1 over the drop, trying to avoid bubble formation fig. 3.2, step 2 . The fluid should not leak out from under the edges of the cover glass. If it does, wait until it dries before sealing. 3. If you examine the slide immediately, you need not seal the coverslip....

Providencia Stuartii Characteristics Alpha Hemolysis

Providencia Alcalifaciens

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

Is Bacterial Sporulation A Reproductive Process Explain

Bacterial Capsule Images

Record and diagram your observations. Describe the arrangement of flagella you observed 1. Why must special stains be used to visualize bacterial capsules, flagella, and endospores 2. Why is it important to know whether or not bacterial cells possess capsules, flagella, or endospores 3. What do endospore stains have in common with the Ziehl-Neelsen acid-fast stain Morello-Mizer-Granato I I. Basic Techniques of I 3. Differential Stains I I The McGraw-Hill Laboratory Manual and Microbiology...

Exercise 8Culture Media

Aseptic Technique Pouring

Once the microscopic morphology and staining characteristics of a microorganism present in a clinical specimen are known, the microbiologist can make appropriate decisions as to how it should be cultivated and what biological properties must be demonstrated to identify it fully. First, a suitable culture medium must be provided, and it must contain the nutrients essential for the growth of the microorganism to be studied see Exercise 2 . Most media designed for the initial growth and isolation...