FIGURE 1.5 (A) Base-peak reconstructed ion chromatogram from a mixture of five standard peptides from a 20 femtomole/peptide on-column injection using gradient elution from a 75-|im-ID tip column as shown Figure 1.4. (B) MS spectrum of peak number 1 showing a doubly charged molecular ion of high S/N. (C) MS/MS spectrum of peak number 3 showing labeled N-terminal (a, b) and C-terminal (y) fragment ions. Scale expansion factors are denoted at the top of the figure. (Data courtesy of William S. Lane, Harvard University.)

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