Role of Lipid Microdomains in the Formation of Supramolecular Protein Complexes and Transmembrane Signaling 141

Gyorgy Vamosi, Andrea Bodnar, Gyorgy Vereb, Janos Szollosi, and Sandor Damjanovich

7.1 Introduction 141

7.1.1 Lateral organization of membrane proteins 142

7.1.2 Factors controlling the organization of membrane proteins 143

7.2 Biophysical Strategies for Studying the Lateral Organization of Membrane Proteins 144

7.2.1 Determination of Domain Size and Overlap between Fluorescence Distributions using Fluorescence Microscopy 144

7.2.2 Fluorescence Resonance Energy Transfer (FRET) 145

7.2.3 Fluorescence Cross-Correlation Spectroscopy: Analysis of Protein Co-Mobility 147

7.2.4 Atomic Force Microscopy (AFM) 149

7.2.5 Scanning Near-Field Optical Microscopy (SNOM) 149

7.3 The Immunological Synapse 150

7.4 Voltage-Gated K+ Channels in Lipid Rafts: Possible Involvement in Local Regulatory Processes 154

7.5 Cell Fusion as a Tool for Studying Dynamic Behavior of Protein Clusters 155

7.6 Lipid Rafts as Platforms for Cytokine Receptor Assembly and Signaling 156

7.7 Organization and Function of Receptor Tyrosine Kinases is Linked to Lipid Microdomains 162

Acknowledgments 166 Abbreviations 166 References 167

Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

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