Figure 4.3 Model of a 16 S rRNA molecule, showing nine variable regions numbered V1-V9. Each dot represents a nucleotide and its variability across species is indicated by the size of the dot (in five classes). From Neefs et al. (1993) with permission from Oxford University Press.

calibrated by marker samples that contain a mixture of known sequences isolated from a clone library from the same environment.

The 16 S rRNA-targeted DGGE approach has become one of the most popular methods with which to profile microbial communities in the environment. An example of such a study is the work by Roling et al. (2001). These authors were interested in microbial communities of groundwater and their potential to degrade aromatic compounds (benzene, toluene, xylene), leaching from a landfill. Groundwater samples

Similarity (%)

0 0

Post a comment