Gtac

CGATTAGC

CGATTAGC

CGATTAGC

CGATTAGC

CGATTAGC

SAGE has developed into a well-established methodology with its own community that organizes workshops, exchanges experiences, and maintains a website (www.sagenet.org). The great advantage of SAGE is that it is very sensitive and performs better than microarray analysis when identifying genes with low expression levels (Gibson and Muse 2002). Applications at the moment are mostly in the medical sector (cancer research) and yeast transcriptomics; ecological applications are still rare (but see Chapter 5). A disadvantage of SAGE is that it relies on massive sequencing, which may be prohibitive to some laboratories, and the recognition of tags requires knowledge about the genome.

2.3.4 Quantitative PCR

In a normal PCR, the amplification product is only a qualitative indicator of the template; the amount of DNA produced after a certain number of cycles, which is usually assessed on a gel, is hardly dependent on the initial amount of template. To make the PCR quantitative, systems have been

Cycle
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