Serological Typing ofMHC Class I Markers

Multiparous women produce antibodies to class I markers present on the fetus. The reactivity of these antibodies have been defined over the years. Antibody panels with known class I allelic specificity have been developed. In the assay, cells are reacted with apanel of antibodies in the presence of complement. Cells are then stained with ethidium bromide to differentiate between live and dead cells. Dead cells stain red whereas live cells stain green. The cells are scored on the basis ofthe percent cells killed (Table 5).

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