Reactivation

The term multiplicity reactivation is applied to the production of infectious virus by a cell infected with two or more virus particles of the same strain, each of which has suffered a lethal mutation in a different gene. Multiplicity reactivation could theoretically lead to the production of infectious virus if animals were to be inoculated with vaccines produced by UV irradiation or treatment with certain chemicals; accordingly these methods of inactivation are not used for vaccine production. Cross-rcactwatwn or marker rescue are terms used to describe genetic recombination between an infectious virus and an inactivated virus of a related but distinguishable genotype, or a fragment of DNA from such a virus; (hey are useful techniques for the experimental virologist

0 0

Post a comment