Basic Principles and Routine Practice Yanbin Dong and Haidong

Mobility Shift Sscp

Single-strand conformational polymorphism SSCP analysis is a simple and sensitive technique for mutation detection and genotyping. The principle of SSCP analysis is based on the fact that single-stranded DNA has a defined conformation. Altered conformation due to a single base change in the sequence can cause single-stranded DNA to migrate differently under nondenaturing electrophoresis conditions. Therefore wild-type and mutant DNA samples display different band patterns. SSCP analysis...

Denaturing High Performance Liquid Chromatography Using the Wave Dna Fragment Analysis System

Heteroduplex Dna

Denaturing high-performance liquid chromatography DHPLC is a chromatographic mutation analysis technique that is based on temperature-dependent separation of DNA containing mismatched base pairs from polymerase chain reaction PCR -amplified DNA fragments. The WAVE system, developed for DHPLC analysis, allows for unattended analysis of 96 samples directly from a PCR plate under a number of different conditions. It utilizes a Peltier cooling platform to maintain sample integrity. Sample detection...