TCell Receptors Were Isolated by Using Clonotypic Antibodies

Identification and isolation of the T-cell receptor was accomplished by producing large numbers of monoclonal antibodies to various T-cell clones and then screening the antibodies to find one that was clone specific, or clonotypic. This approach assumes that, since the T-cell receptor is specific for both an antigen and an MHC molecule, there should be significant structural differences in the receptor from clone to clone; each T-cell clone should have an antigenic marker similar to the idiotype markers that characterize monoclonal antibodies. Using this approach, researchers in the early 1980s isolated the receptor and found that it was a het-erodimer consisting of a and (3 chains.

When antisera were prepared using a(3 heterodimers isolated from membranes of various T-cell clones, some antis-era bound to a (3 heterodimers from all the clones, whereas other antisera were clone specific. This finding suggested that the amino acid sequences of the TCR a and (3 chains, like those of the immunoglobulin heavy and light chains, have constant and variable regions. Later, a second type of TCR heterodimer consisting of 8 and 7 chains was identified. In human and mouse, the majority of T cells express the a (3 heterodimer; the remaining T cells express the 78 heterodimer. As described below, the exact proportion of T cells expressing a (3 or 78 TCRs differs by organ and species, but a (3 T cells normally predominate.

Was this article helpful?

0 0
How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book

Post a comment