KnockIn Technology Allows the Replacement of an Endogenous Gene

In addition to deleting a gene of choice, it also is possible to replace the endogenous gene with a mutated form of that gene. As in the strategy for knocking out a gene, DNA constructs that carry mutations in a particular gene can be exchanged for the endogenous gene. It also is possible to replace all of an endogenous gene with a DNA sequence of choice. In a recent report, for example, the CD4 gene was replaced with the one for p-galactosidase. In these experiments, the CD4 promoter was left intact to drive the expression of p-galactosidase, which catalyzes the color change of certain reporter chemicals to blue. Because the CD4 promoter drove the expression of p-galactosidase, only those thymic cells destined to express CD4 turned blue in the presence of the reporter chemicals. Data from these experiments were useful in tracing CD4/CD8 lineage commitment in developing T cells.

Was this article helpful?

0 0
Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

Get My Free Ebook

Post a comment