Hemagglutination Is Used in Blood Typing

Agglutination reactions (Figure 6-7) are routinely performed to type red blood cells (RBCs). In typing for the ABO

FIGURE 6-7

Demonstration of hemagglutination using antibodies against sheep red blood cells (SRBCs). The control tube (10) contains only SRBCs, which settle into a solid "button." The experimental tubes 1-9 contain a constant number of SRBCs plus serial two-fold dilutions of anti-SRBC serum. The spread pattern in the experimental series indicates positive hemagglutination through tube 3. [Louisiana State University Medical Center/MIP. Courtesy of Harriet C. W. Thompson.]

FIGURE 6-7

Demonstration of hemagglutination using antibodies against sheep red blood cells (SRBCs). The control tube (10) contains only SRBCs, which settle into a solid "button." The experimental tubes 1-9 contain a constant number of SRBCs plus serial two-fold dilutions of anti-SRBC serum. The spread pattern in the experimental series indicates positive hemagglutination through tube 3. [Louisiana State University Medical Center/MIP. Courtesy of Harriet C. W. Thompson.]

antigens, RBCs are mixed on a slide with antisera to the A or B blood-group antigens. If the antigen is present on the cells, they agglutinate, forming a visible clump on the slide. Determination of which antigens are present on donor and recipient RBCs is the basis for matching blood types for transfusions.

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Responses

  • semira
    Why are sheep red blood cells used for hemagglutination?
    8 years ago
  • ashlyn mcintosh
    How is hemagglutination used in blood typing?
    7 years ago

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