Tions and increase background noise on the hybridized array by causing nonspecific hybridization to the glass surface

1. 100% ethanol (BD; cat. no. 10107) Store at RT; stable for minimum 5 yr. Safety information: highly flammable. Keep away from sources of ignition. Keep container tightly closed.

3. Microfuge suitable for 2-mL Microfuge tubes.

4. RNeasy mini kit (X50; Qiagen; cat. no. 74104) Store at RT; stable for 1 yr) The kit contains:

1. RLT buffer. (Safety information: RLT buffer contains guanidine isothiocyanate, harmful by inhalation, in contact with skin and if swallowed. Contact with acids liberates very toxic gas.)

2. RPE buffer. (Supplied as a concentrate. Before using for the first time add 4 volumes of ethanol [96-100%] as indicated on the bottle. Safety information: Flammable.)

3. RNeasy mini spin columns (silica gel-based membranes, binding up to 100 |j.g of RNA longer than 200 bases)

4. 1.5-mL collection tubes.

5. 2.0-mL collection tubes.

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