This step is performed to eliminate unincorporated biotinlabeled ribonucleotides

1. 100 % ethanol (BD; cat. no. 10107). Store at RT, stable for minimum 5 yr. Safety information: highly flammable. Keep away from sources of ignition. Keep container tightly closed.

3. RNeasy Mini Kit (X50; Qiagen; cat. no. 74104). Store at RT, stable for 1 yr. The kit contains:

RLT buffer. (Safety Information: RLT Buffer contains guanidine isothiocyanate, harmful by inhalation, in contact with skin and if swallowed. Contact with acids liberates very toxic gas.)

RPE buffer. (The buffer is supplied as a concentrate. Before using for the first time, add 4 volumes of ethanol (96-100%) as indicated on the bottle. Safety information: flammable.)

4. RNeasy mini spin columns. (The columns contain a silica-gel-based membrane, which binds up to 100 mg of RNA longer than 200 base pairs.)

5. 1.5-mL collection tubes.

6. 2.0-mL collection tubes.

7. Microfuge suitable for 2-mL tubes.

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