The original SEREX technique relied on the detection of antigens expressed by a tumor-derived cDNA library screened with autologous serum (i.e., derived from the same patient as the tumor) (15). Subsequently, a number of variations of this technique have been developed and these are discussed where appropriate. There is a SEREX database (http://www.licr.org/SEREX.html) that is maintained by the "SEREX core group." This site provides information on sequences obtained using SEREX and links to other research groups using this technique. Both DNA and protein sequences can be searched through this database to investigate whether they represent a known antigen previously detected using the SEREX technique.
Was this article helpful?