RNA degradation is one of the principal problems and frustrations of gene expression profiling. To prevent RNase contamination, always wear latex or vinyl gloves and use diethyl pyrocarbonate (DEPC)-treated distilled water (see below), sterile RNase-free disposable pipet tips, and Microfuge tubes throughout the procedure. Avoid sources of dust or other contaminants, which can interfere with the multiple procedures you will be undertaking.
2. RNAse-free tips for 2-, 20-, 200-, and 1000-pL pipets.
3. RNAse-free 0.5-, 1.5-, and 2-mL Microfuge tubes.
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