Preventing RNA Degradation and Contamination

RNA degradation is one of the principal problems and frustrations of gene expression profiling. To prevent RNase contamination, always wear latex or vinyl gloves and use diethyl pyrocarbonate (DEPC)-treated distilled water (see below), sterile RNase-free disposable pipet tips, and Microfuge tubes throughout the procedure. Avoid sources of dust or other contaminants, which can interfere with the multiple procedures you will be undertaking.

2. RNAse-free tips for 2-, 20-, 200-, and 1000-pL pipets.

3. RNAse-free 0.5-, 1.5-, and 2-mL Microfuge tubes.

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    How to prevent rna degradation?
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