PCR Gel Electrophoresis and End Labeling

1. Taq DNA polymerase (Perkin-Elmer Biosystem; Milano, Italy).

2. Mastercycler 330 (Eppendorf Scientific, Hamburg, Germany).

3. T4 polynucleotide kinase (Gibco); store at -20°C. Do not leave at RT or at 4°C for prolonged periods!

4. y 32P-dATP (Amersham); store at -20°C, radiation hazard, use special protections to avoid radioisotope contamination.

8. Acetic acid (Fluka); store at RT. Highly corrosive; wear gloves while handling.

9. Acrylamide:bisacrylamide 19:1 (Fluka). Reconstitute in deionized water by stirring overnight and store at 4°C.Light sensitive and toxic; wear glove while handling it.

10. 68% HNO3 analytical reagent (Prolabo; France).

11. AgNO3 ACS reagent (Sigma). Light sensitive; wear gloves to protect skin.

13. 37% Formaldeide (Sigma), toxic.

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