Antibodies are invaluable tools for use in relatively simple, rapid, and reproducible techniques to investigate protein expression in both normal and neoplas-tic cells. In addition to the subcellular localization of proteins, antibodies provide important information concerning changes in protein levels and distribution that occur within the cell during normal cellular differentiation or as part of an oncogenic process. This chapter describes techniques using antibodies that can be used for the study of ALK-positive ALCL.

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