In addition to the reactives here described, a tabletop microcentrifuge, wet ice, 1.6-mL polypropylene Eppendorf tubes, and a thermal bath equilibrated at 65 °C also are needed.
2. GlassMAX spin cartridges (also included in the kit).
3. 1X Wash buffer. Before using the system for the first time, the wash buffer must be prepared from the buffer concentrate supplied in the kit: Pipet 1 mL of the concentrate into a 50-mL Falcon tube, and then add 18 mL of deionized water and 21 mL of absolute ethanol, mixing thoroughly.
Was this article helpful?