Flow Cytometry

1. Ammonium Chloride Lysing Solution: 0.86 g of ammonium chloride in 1 L of distilled water. Do not adjust pH. Stable for 1 wk.

2. Wash Buffer: FACSFlow (BD Biosciences, UK) containing 1% w/v bovine serum albumin (Sigma, Poole, UK).

3. Suggested Flow Cytometry Panel for Surface Antigens

1. Ammonium Chloride Lysing Solution: 0.86 g of ammonium chloride in 1 L of distilled water. Do not adjust pH. Stable for 1 wk.

2. Wash Buffer: FACSFlow (BD Biosciences, UK) containing 1% w/v bovine serum albumin (Sigma, Poole, UK).

3. Suggested Flow Cytometry Panel for Surface Antigens

FITC

PEe

PEe:Cy5

rn3J

CD3á

CD19<i

CD206

CD5á

CD19á

CD10¿

CD38"

CD19á

Kappaá

Lambda^

CD19á

FMC7C

CD22"

CD19á

CD11aá

CD23á

CD19á

IgM"

IgD"

CD19á

IgG"

CD79b6

CD19á

"BP Biosciences._

^Coulter/Immunotech. cSilenus.

^In-house reagent. ePE, phycoerythrin.

"BP Biosciences._

^Coulter/Immunotech. cSilenus.

^In-house reagent. ePE, phycoerythrin.

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