1. Distilled water.
2. DEPC (Sigma-Aldrich; cat. no. 15922). Store at 4-8°C; stable for 2 yr. Safety information: DEPC is a suspected carcinogen and should be handled with great care. Toxic if inhaled, in contact with skin, and if swallowed. Keep away from food and drink. Irritating to eyes, respiratory system. Combustible.)
To prepare DEPC H2O, add 0.1 mL of DEPC to 100 mL of distilled water or 1 mL of DEPC to 1000 mL of distilled water. Shake vigorously to bring DEPC into solution, then incubate for 12 h at 37°C. Autoclave for 15 min to remove any trace of DEPC. Store, shielded from light at room temperature (RT). Stable for months.
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