Denaturing the Fragmented Target in the Hybridization Cocktail

Remember that only four chips can be run simultaneously in each fluidics station you have access to. Because the post target-probe hybridization chip can only be kept for 3 h after completion of the hybridization procedure, don't denature more sample hybridization cocktails than can be taken through the fluidics stages in two consecutive batches (each run will take about 2 h); see Note 56).

1. Turn on two heating blocks set at 99°C and 45°C.

2. Defrost any frozen hybridization cocktails.

3. Incubate the hybridization cocktails for 5 min at 99°C.

4. Transfer the cocktails immediately to the heating block set at 45°C for 5 min.

5. Spin at maximum for 5 min to precipitate any insoluble material.

Was this article helpful?

0 0

Post a comment