Concentration and Cleaning of Total RNA by Ethanol Precipitation

1. Isopropanol (BD; cat. no. 10224). Store at room temperature; stable for minimum 5 yr. Safety information: highly flammable. Keep away from sources of ignition. Keep container tightly closed. May cause harm to the unborn child. Irritating to eyes.

2. 3 M sodium acetate, pH 5.2 (Sigma-Aldrich; cat. no. 57899). Store at RT; stable for 1 yr. Safety information: avoid contact and inhalation.

3. 100% ethanol (BD; cat. no. 10107). Store at RT; stable for minimum 5 yr. Safety information: highly flammable. Keep away from sources of ignition. Keep container tightly closed.)

5. 80% ethanol (make up using 100% Ethanol and DEPC H2O)

5. 1.5-mL Microfuge tubes.

6. Microfuge.

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