CleanUp of dscDNA

1. Phase Lock Gel tubes (2 mL, light; Eppendorf; cat. no. 0032 005101). Store at RT, stable for 1 yr.

2. Phenol:chloroform:isoamyl alcohol, 25:24:1, pH 6.6, buffered pH 7.9 (Ambion; cat. no. 9732). Store at 4°C or -20°C; stable for 6 mo. Accompanied by a separate pre-made Alkaline Buffer solution to be added just prior to use (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Safety Information: toxic in contact with skin and if swallowed; causes burns.)

3. 1.5-mL Microfuge tubes.

4. Microfuge suitable for 2-mL tubes.

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