1. Ficoll-Hypaque (Seromed, Biochrom AG; Berlin, Germany); store at 4°C.
2. Percoll™ (Amersham Pharmacia Biotech; Uppsala, Sweden); store unopened at room temperature (RT).
4. Fetal calf serum (Seromed); deplete of complement by heating 1 h at 56°C, aliquot, and store frozen at -20°C.
5. Glutamine, Penstreptomycin, and Na pyruvate (Seromed); store frozen.
6. Neuraminidase (Dade Behring; Milano, Italy).
7. 10X Hanks Balanced Salt Solution (Gibco BRL; Paisley, Scotland); store unopened at RT.
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