Imaging Of Microbes

Recent advances in optical imaging have dramatically expanded the capabilities of light microscopes and their usefulness in microbiology research to study the structure and functions of small microbes. Some of these advances include improved fluorescent probes, better cameras, new techniques such as confocal and near-field microscopy, and the use of computers in imaging and image analysis. These new technologies have now been applied to microbial investigations with great success (Fung and Theriot, 1998). This section provides selected examples of imaging of microbes that are comparable to or, in the case of viruses, significantly smaller than the wavelength of light used for imaging. The examples provided use both confocal and near-field microscopic techniques.

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