Imaging of Nucleic acids

Flow Cytometry Acridine Orange Lin

Nucleic acid stains are usually cationic dyes that bind with the polyanionic nucleic acid, thus showing specificity for DNA and RNA. Furthermore, hydrophilic hydrophobic interactions play an important role in a providing some dyes with the ability to intercalate between the nucleic acid bases and b providing other dyes with the ability to bind with double-stranded nucleic acids in grooves. In double-stranded DNA, the alignment of the strands is antiparallel and asymmetric along the axis. This...

The Components of a Flow Cytometer

Flow Cytometry Flow Cell Pictures

A flow cytometer consists of a light source, which in all modern flow cytome-ters is a laser, and illumination optics to focus the laser beam on to a flowing biological cell or a polystyrene microsphere, which is fluorescently labeled. The scattered laser light and the fluorescence response are separated and focused onto photodetectors. A special electronics processes the optical response and controls a sorter if it is provided. These components of a flow cytometer are described in detail here....

Laser Tissue Contouring And Restructuring

The two specific applications discussed here are for the use of lasers in der-matologic and ophthalmologic procedures. The theory of selective photother-molysis, introduced by Anderson and Parrish in 1981, is the basis for much advancement in dermatological lasers Anderson and Parrish, 1983 . It allows for highly localized destruction of light absorbing targets in skin, with minimal damage to the surrounding tissue. To achieve selective photother- molysis, an appropriate wavelength, exposure...