Preparation of Activated Solid Phases

2.1.1. Preparation of Thiol-Agarose and Titration of Thiol Groups

1. Sepharose 4B (Pharmacia BTG, Uppsala, Sweden).

2. Epichlorohydrin (1-chloro-2,3-epoxypropane) (Sigma).

3. Sodium thiosulfate (Fluka AG, Buchs, Switzerland).

4. Dithiothreitol (DTT) (Sigma).

2.1.2. Preparation of 2-Pyridyldisulfide Agarose and Titration of Pyridyl Disulfide Groups

1. Mercapto pyridine (Fluka AG, Buchs, Switzerland).

2.1.3. Preparation of Thiolsulfonate-Agarose (TS-gel)

1. Perhydrol® (30% hydrogen peroxide) (Merck AG, Darmstadt, Germany).

2.1.4. Preparation of Thiolsulfinate-Agarose (TSI-gel).

1. Magnesium monoperoxyphthalate (MMPP, Merck AG).

2. Potassium ferricyanide (Baker, Phillipsburg, NJ).

2.1.5. Titration of Thiol-Reactive Structures in the Agarose Derivatives

1. Reduced glutathione (Sigma).

2. 2,2'-Dipyridyldisulfide (2-PDS, Sigma).

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