Info

Epoxy supports, see Amino-epoxy-

Sepabead®; Histidine-tagged enzyme immobilization; Multipoint covalent immobilization Ethanol, production with Lentikats-encapsulated yeast, 342

Ferredoxin-NADP reductase, gold support immobilization, 236 Fluidized bed reactors, advantages, 312, 314 examples of immobilized systems, 312, 313

magnetic particles, 314 pullulanase and glucoamylase mixed immobilization system, activity assays, 315 alginate bead entrapment, 315, 316 materials, 314-316 starch hydrolysis, 315-317 Formate dehydrogenase, cross-linked enzyme aggregates, 42

Galactose oxidase, cross-linked enzyme aggregates, 42 P-Galactosidase,

V-acetyllactosamine synthesis in ionic liquids, 264-267 aldehyde-dextran cross-linking stabilization, 134, 135 amino-epoxy-Sepabead® immobilization,

158, 159 concanavalin A immobilization, activity assay, 111, 112 conjugation and immobilization on

Sephadex matrix, 112, 113 materials, 111, 112 cross-linked enzyme aggregates, 42, 43 gold support immobilization of choline-binding domain fusion protein, activity assay, 234, 235, 237 gold electrode terminal amino group activation, 234 hydrophilic spacer coupling, 234 protein binding, 234 reversibility of binding, 235 thiocholine coupling to electrode, 234

one-step purification, immobilization, and stabilization of histidine-tagged enzyme on metal chelate-epoxy supports, 123125, 127

polyethyleneimine ionic polymer support immobilization, 210 thiol-reactive agarose immobilization, E. coli enzyme, activity assay, 196, 199 elution of enzyme, 197, 200 glutathione blocking, 197, 200 immobilization, 197, 199, 200 protein assay, 196, 199 K. lactis enzyme, activity assay, 197 elution of enzyme, 198, 201 enzyme reduction, 197, 198, 200 glutathione blocking, 198, 200 immobilization, 198, 200 lactose hydrolysis, 198, 201 protein assay, 197 recycling, 199, 201, 203

Gasoline hydrocarbons, bacteria encapsulation for bioremediation, bacteria incorporation, 421, 422 canola oil preparation, 421, 422 cell harvesting, 421 emulsion techniques, 418, 419, 421, 422

enrichment culture, 421 gelation, 422 gellan gum structure, 420 materials, 419, 420 microsphere separation, 422 overview, 416-418 pregel solution preparation, 421 components, 415, 416 Gellan gum, see Gasoline hydrocarbons Glucoamylase, fluidized bed reactor pullulanase mixed immobilization system, activity assays, 315 alginate bead entrapment, 315, 316 materials, 314-316 starch hydrolysis, 315-317 Glucose oxidase, cross-linked enzyme aggregates, 42 cross-linked imprinting, bioconversion of glucose or galactose, 181-183 cross-linking, 180-182 derivatization, 180, 181 imprinting, 180 materials, 179, 180 immobilization on preactivated agarose, 60

Glutaraldehyde, d-amino acid oxidase immobilization, 60

cation-adsorbed protein immobilization, adsorption, 59, 63 desorption, 60, 63

immobilization reaction, 59, 60, 63 materials, 59 principles, 58, 59 glucose oxidase immobilization on preactivated agarose, 60 glutaryl acylase immobilization, 60, 61

protein immobilization on preactivated animated supports, immobilization reaction, 59, 63 materials, 59 principles, 58 support activation, 59, 62 Glutaryl acylase, amino-epoxy-Sepabead® immobilization, 159, 161

immobilization using glutaraldehyde, 60, 61

multipoint covalent immobilization on glyoxyl supports, amination of enzyme, 168, 171 immobilization, 169, 171 structural stabilization, 169, 170 one-step purification, immobilization, and stabilization of histidine-tagged enzyme on metal chelate-epoxy supports, 121, 123, 127

Glycoproteins, see Concanavalin A Glyoxyl supports, multipoint covalent immobilization, advantages and limitations, 164 amination of enzyme, 165, 170 examples, 163, 164 glutaryl acylase, amination of enzyme, 168, 171 immobilization, 169, 171 structural stabilization, 169, 170 immobilization, 166, 171 materials, 164, 165, 170 multipoint covalent attachment, 166, 171 penicillin G acylase, amination of enzyme, 166 immobilization, 166, 167, 171 structural stabilization, 167 support preparation, agarose gel activation, 165, 171 glyoxyl-agarose preparation, 165, 171 Gold supports, bimolecular interaction detection, 230 histidine-tagged proteins, activity assay, 236, 237 ferredoxin-NADP reductase immobilization, 236 nitriloacetic acid metal chelating, 235

reversibility of binding, 236 thioctic acid amidation, 235 protein A-immunoglobulin G coupling, 229

protein interactions, 230 self-assembled monolayers and protein immobilization, carboxylic acid group modification,

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