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Note: The numbers express the amount of detergent necessary to desorb 100% of lipase from the support.

Note: The numbers express the amount of detergent necessary to desorb 100% of lipase from the support.

evant conditions, it is convenient that the enzyme can, after enzyme deactivation, be desorbed from the support in order to reload the support with fresh enzyme and take advantage of the reversibility of the technique. Table 1 shows that the adsorbed lipases on octadecyl-Sepabeads or octyl-agarose could be fully desorbed by using moderate concentrations of detergent. Moreover, the incubations with urea and guanidine enable the full desorption of the lipase.

The regenerated supports could be used to immobilize new batches of lipase without significant differences in terms of loading capacity compared to the first immobilization.

4. Notes

1. To take suspension the tips have to be cut.

2. In some instances, if immobilization is slow, it may be convenient to dilute the lipase preparation at low ionic strength (even adding some glycerol).

3. Check that desorption of the lipase caused by organic solvents is not produced.

4. It is first necessary to check the effect of a similar amount of organic solvent during the assay in the enzyme activity; organic solvents may be inhibitors or activator of the lipases.


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