Info

Acetate buffer 50 mM, pH 5.0. Flow and batch notations are referred to flow and batch calo-rimetry, respectively. Nis the number of the binding sites. (From ref. 18. Copyright 2005, with permission from Elsevier.)

Acetate buffer 50 mM, pH 5.0. Flow and batch notations are referred to flow and batch calo-rimetry, respectively. Nis the number of the binding sites. (From ref. 18. Copyright 2005, with permission from Elsevier.)

1.3. Adsorption: Thermal Unfolding

1.3.1. DSC Results

Inert supports can be used as protein and enzymes carriers for particular applications such as reactions in non aqueous solvent systems. Depending upon the characteristics of the support, the adsorption interactions may be based on weak interaction forces, either electrostatic or of London type (i.e., van der Waals, dipoledipole, hydrophobic). Celite is an essentially uncharged diatomaceous earth material characterized by low water-affinity. Unlike other common adsorbing material, such as synthetic polymeric resins, celite does not induce any major structural modification on adsorbed RNase (23). The thermal unfolding of RNase adsorbed on celite in the presence of dodecane as solvent is shown in Fig. 5A at a water content of 0.48 gram per gram (g/g) of protein. As can be seen, the melting transition is clearly observed in these conditions. The transition strongly depends upon

Lite Immobilisation

Fig. 5. DSC thermogram of the unfolding of RNase adsorbed on celite. (A) Direct calo-rimetric output in the presence of dodecane, water content 0.48 g/g. (B) Excess heat capacity as a function of the temperature in the presence of dodecane, water content 1.1 g/g (A), 0.48 g/g (b), 0.22 g/g (C). (From ref. 25. Copyright 2005, American Chemical Society.)

Fig. 5. DSC thermogram of the unfolding of RNase adsorbed on celite. (A) Direct calo-rimetric output in the presence of dodecane, water content 0.48 g/g. (B) Excess heat capacity as a function of the temperature in the presence of dodecane, water content 1.1 g/g (A), 0.48 g/g (b), 0.22 g/g (C). (From ref. 25. Copyright 2005, American Chemical Society.)

Table 3

Thermodynamic Parameters of RNase Unfolding in Phosphate Buffer 10 mM, pH 7.0

Table 3

Thermodynamic Parameters of RNase Unfolding in Phosphate Buffer 10 mM, pH 7.0

Was this article helpful?

0 0
Diabetes Sustenance

Diabetes Sustenance

Get All The Support And Guidance You Need To Be A Success At Dealing With Diabetes The Healthy Way. This Book Is One Of The Most Valuable Resources In The World When It Comes To Learning How Nutritional Supplements Can Control Sugar Levels.

Get My Free Ebook


Post a comment