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Biomass concentration in test samples is calculated by using response (R) in RLU values (the baseline signal is subtracted from that measured upon addition of the ATP monitoring reagent). The formula to calculate the biomass concentration in samples containing immobilized cells is as follows:

Biomass conc g mL

where S is standard concentration in mg/mL equivalent to the Response value of the sample (based on the calibration line) and m is weight of the gel beads in grams (g).

3.3. Calculation of Adenosine Triphosphate Content

1. Samples can be analyzed for concentration of intracellular ATP using the ATP standard contained in the kit. Note: The ATP standard must be further diluted before use! See instructions for more information!

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