aAfter each use, elution was carried out and then the gel was regenerated.

aAfter each use, elution was carried out and then the gel was regenerated.

33. In order to demonstrate the feasibility of reusing the support, several cycles of immobilization, elution of bound protein, regeneration of thiol-reactive adsorbent (TSI-gel), and reuse is performed. As high amounts of a model thiol-protein are required, bovine serum albumine-SH, provided with 4.0 moles of de novo thiol groups per mole of protein through chemical modification with SPDP, is selected for this purpose (Table 5).


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