Immobilization of LAsparaginase Into Liposomes

2.2.1. Preparation of i-Asparaginase-ioaded iiposomes

1. Egg phospatidylcholine, cholesterol, and stearylamine (Sigma).

2. L-Asparaginase (Sigma).

3. Mannitol (Sigma).

4. NaCl (Panreac Quimica).

2.2.2. Characterization of L-Asparaginase-Loaded Liposomes

3. 66 mMPhosphate buffer, pH 7.4.

5. Anhydrous glycerol (Sigma).

7. a-Ketoglutarate-sodium, L-glutamic oxaloacetate transaminase, L-malic dehydrogenase, NADH, and L-asparagine (Sigma).

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