Contents

Preface v

Contributors xi

1 Immobilization of Enzymes as the 21st Century Begins:

An Already Solved Problem or Still an Exciting Challenge? Jose M. Guisan 1

2 Immobilization of Enzymes: A Literature Survey

Beatriz M. Brena and Francisco Batista-Viera 15

3 Cross-Linked Enzyme Aggregates

Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen 31

4 Immobilization-Stabilization of Enzymes by Multipoint Covalent

Attachment on Supports Activated With Epoxy Groups Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente 47

5 Glutaraldehyde in Protein Immobilization: A Versatile Reagent Lorena Betancor, Fernando Lopez-Gallego, Noelia Alonso-Morales,

Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan 57

6 Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques Manfred T. Reetz 65

7 Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques Monica Campas and Jean-Louis Marty 77

8 Design of Smart Biocatalysts:

Immobilization of Enzymes on Smart Polymers Ipsita Roy andMunishwar N. Gupta 87

9 Affinity Immobilization of Tagged Enzymes

Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty 97

10 Bioaffinity Immobilization

Ipsita Roy and Munishwar N. Gupta 107

11 One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu,

Rodrigo Torres, Fernando Lopez-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente 117

viii Contents

12 Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan 129

13 Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan 143

14 Immobilization and Stabilization of Proteins by Multipoint Covalent

Attachment on Novel Amino-Epoxy-Sepabeads® Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu,

Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes,

Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor,

Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian,

Jose M. Guisan, and Roberto Fernandez-Lafuente 153

15 Improved Stabilization of Chemically Aminated Enzymes Via

Multipoint Covalent Attachment on Glyoxyl Supports Tamara Montes, Fernando López-Gallego, Manuel Fuentes,

Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente 163

16 Stabilization of New Imprint Property of Glucose Oxidase in Pure

Aqueous Medium by Cross-Linked-Imprinting Approach Alankar Vaidya and Lutz Fischer 175

17 Reversible Covalent Immobilization of Enzymes

Via Their Thiol Groups Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta 185

18 Very Strong But Reversible Immobilization of Enzymes on Supports

Coated With Ionic Polymers Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente 205

19 Immobilization of Enzymes on Magnetic Particles Martina Koneracká, Peter Koptéansky, Milan Timko,

Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira 217

20 Immobilization of Proteins on Gold Surfaces

José M. Abad, Marcos Pita, and Víctor M. Fernández 229

21 Immobilization of Enzymes on Electrodes

Gilvanda Silva Nunes and Jean-Louis Marty 239

Contents ix

22 Immobilization of Enzymes for Use in Organic Media

Patrick Adlercreutz 251

23 Immobilization of Enzymes for Use in Ionic Liquids

Pedro Lozano, Teresa de Diego, and José L. Iborra 257

24 Immobilization of Enzymes for Use in Supercritical Fluids

Pedro Lozano, Teresa de Diego, and José L. Iborra 269

25 Immobilized Enzymes for Biomedical Applications

Amaia Esquisabel, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz 283

26 Characterization of Immobilized Enzymes by Microcalorimetry

Ezio Battistel and Giovanni Rialdi 295

27 Use of Immobilized Biocatalysts in Fluidized Bed Format

Ipsita Roy and Munishwar N. Gupta 311

28 Taylor-Couette Vortex Flow in Enzymatic Reactors

Roberto Campos Giordano and Raquel de Lima Camargo Giordano 321

29 A Novel Immobilization Method for Entrapment: LentiKats®

Marc Schlieker and Klaus-Dieter Vorlop 333

30 Encapsulation of Cells in Alginate Gels

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz 345

31 Immobilization of Cells on Polyurethane Foam

Ignacio de Ory, Gema Cabrera, Martin Ramirez, and Ana Blandino 357

32 Immobilization of Cells With Transition Metal

Pedro Fernandes 367

33 Immobilization of Microalgae

Nirupama Mallick 373

34 Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications Marián Navrátil, Juraj Svitel, Peter Gemeiner 393

35 A Proteomic Approach to Biofilm Cell Physiology

Laurent Coquet, Sébastien Vilain, Pascal Cosette, Thierry Jouenne, and Guy-Alain Junter 403

36 Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons Peyman Moslemy, Serge R. Guiot, and Ronald J. Neufeld 415

37 Biomedical Applications of Immobilized Cells

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz 427

Index 439

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