MEF cells from commercial sources

There are several commercial sources for MEFs from various mouse strains and containing various selectable drug-resistant markers. Depending on the level of use, this can be a convenient and economical alternative to the de novo preparation of MEF feeder layers.

Fibroblast Feeder Layer

Figure 3.1 Low (A) and high (B) magnification photomicrographs showing morphology of MEF feeder layers. MEFs do not form the whorls of cells that are typical of other fibroblasts used as feeder layers, such as human foreskin fibroblasts. (C) High density hESC (WA09 line) colonies cultured on MEF feeder layers. Photos by Dr Maria Baracova.

Figure 3.1 Low (A) and high (B) magnification photomicrographs showing morphology of MEF feeder layers. MEFs do not form the whorls of cells that are typical of other fibroblasts used as feeder layers, such as human foreskin fibroblasts. (C) High density hESC (WA09 line) colonies cultured on MEF feeder layers. Photos by Dr Maria Baracova.

■ ATCC (www.atcc.org)

■ Chemicon International (www.chemicon.com)

■ Primogenix, Inc. (www.primogenix.com)

■ Stem Cell Technologies (www.stemcell.com)

■ Globalstem, Inc (www.globalstem.com).

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